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In the Amazon, kambo is typically gathered early in the morning, when the Phyllomedusa bicolor-- the huge monkey frog from which the secretion is derived-- are singing and can easily be discovered. A number of peptides in Kambo are most likely accountable for the effect.

The Kambo peptide dermorphin increased the production of prolactin in healthy 28 human topics. Kambo is normally harvested from the tree frog early in the morning when a tribal individual follows the tune of the frog. When captured, the frog is connected in an "x" shape and the poisonous secretions are thoroughly scraped from their legs and stored on wooden sticks for usage in the Kambo event.