About -ADB absence in the examined urine samples was defined in by the reality that solely a small quantity of the analyte in the herbal smoke was inhaled by the donor in a really quick time frame. Yet, it is value noting, that urine, probably the most simply accessible and not so advanced human fluid, may be an inconvenient matrix in the analysis of ionic character drugs as a result of their possible pH variability. According to the available literature, only a few techniques—protein precipitation, liquid–liquid extraction, solid-phase extraction and QuEChERS —are at present employed for analytical procedures of 5F-ADB . Protein precipitation and liquid–liquid extraction, the commonest sample preparation methods employed for 5F-ADB analysis, ensure comparatively quick isolation of the analyte from organic matrices. <br><br>